Abstract Description

Bifidobacterium was first isolated from breast-fed infant feces but has so far been discovered from various ecological niches including sewage and fermented milk. Although Bifidobacteria were often isolated from calf feces, limited information exists on their occurrence and quantity in calf gut. In this study, we report the genome sequence of Bifidobacterium bifidum BB11 strain, isolated from the Holstein bull calf's rumen samples. For the isolation and extraction of genomic DNA from 48h grown cultures, a Quick-DNA fungal/bacterial miniprep kit (Zymo Research, Irvine, CA) was used according to the manufacturer’s instructions. Thereafter, sequencing was performed using the Illumina NextSeq platform. The strain tested positive for probiotic characteristics such as bile salt tolerance, adhesion, and acid tolerance. The genome size was 2,200,000 bp with the guanine and cytosine (G+C) content of 62.5%. The data obtained support for future research regarding Bifidobacterium bifidum, phylogenetics, epidemiologies, mining genes coding for probiotic characteristics such as bile salt tolerance, acid tolerance, and antimicrobial resistance genes.