Abstract Description

Polycyclic Aromatic Hydrocarbons (PAHs) are persistent environmental pollutants that pose risks to ecosystems and human health, due to their mutagenic, carcinogenic and teratogenic properties as well as resistance to traditional degradation. This study will explore endophytic enhanced bioremediation as an alternative remediation method for PAHs. The primary aims of this study is to isolate and characterise PAHs degrading endophytic bacteria from plants growing on a sludge-bed and to determine their efficacy in the degradation of selected PAHs (phenanthrene, naphthalene and pyrene). Healthy plant samples will be collected and surface-sterilized to isolate endophytic bacteria. Obtained strains will be screened for their ability to degrade selected PAHs. For strains demonstrating significant degradation, their degradation pathways and enzyme kinetics will be characterised through enzyme activity assays. By investigating this promising technology, this study will contribute to the development of modern, cost-effective and sustainable sludge treatment methods, while also providing potential solutions for large-scale, low-cost bioremediation practices. Furthermore, findings of this study will support development of green technologies for soil restoration and will benefit communities by promoting safer and cleaner environments.