Abstract Authors
Amahle Bokveld -
Department of Biotechnology and Biological Sciences , Faculty of Science and Agriculture , University of Fort Hare, Alice, 5700, South Africa
SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Alice, South Africa
DSTI/NRF SARChI in Water Quality and Environmental Genomics, University of Fort Hare, Alice, South Africa
E Aladejana -
SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Alice, South Africa
DSTI/NRF SARChI in Water Quality and Environmental Genomics, University of Fort Hare, Alice, South Africa
N Ntozonke -
Department of Biotechnology and Biological Sciences , Faculty of Science and Agriculture , University of Fort Hare, Alice, 5700, South Africa
A I Okoh -
Department of Biotechnology and Biological Sciences , Faculty of Science and Agriculture , University of Fort Hare, Alice, 5700, South Africa
SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Alice, South Africa
DSTI/NRF SARChI in Water Quality and Environmental Genomics, University of Fort Hare, Alice, South Africa
N Nontongana -
Department of Biotechnology and Biological Sciences , Faculty of Science and Agriculture , University of Fort Hare, Alice, 5700, South Africa
SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Alice, 5700, South Africa
DSTI/NRF SARChI in Water Quality and Environmental Genomics, University of Fort Hare, Alice, South Africa
Abstract Description
The increasing understanding of inflammatory pathways has spurred significant interest in developing novel anti-inflammatory strategies. Numerous studies have indicated that traditional medicines have substantial anti-inflammatory effects by modulating important intracellular signalling pathways, making them a promising route. Lipopolysaccharide (LPS) was used to assess the anti-inflammatory activity of the extracts. Sulfanilamide and N-(1-naphthyl) ethylenediamine (NED) solutions were prepared to measure the amount of NO produced. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was used to evaluate cell viability to verify that toxicity was not a contributing factor. AAB showed cytotoxic at 125 and 250 µg/mL, and EAB at 62.5 and 125 µg/mL. Both extracts showed a negative effect on cell viability. With minimal effect on cell viability, the reduction in nitrite content in response to LPS activation of RAW macrophages is indicative of anti-inflammatory activity. A dose-dependent decrease in nitrite concentration is evident with treatment of both extracts, suggesting anti-inflammatory potential. The study suggests that the test samples exhibit anti-inflammatory activity. This research adds to the increasing availability of scientific data regarding the possible therapeutic advantages of traditional medicine methods and emphasises these benefits.
